Cell Type-Specific In Vitro Gene Expression Profiling of Stem Cell-Derived Neural Models

Cell Type-Specific In Vitro Gene Expression Profiling of Stem Cell-Derived Neural Models

Blog Article

Genetic lovesense 3 and genomic studies of brain disease increasingly demonstrate disease-associated interactions between the cell types of the brain.Increasingly complex and more physiologically relevant human-induced pluripotent stem cell (hiPSC)-based models better explore the molecular mechanisms underlying disease but also challenge our ability to resolve cell type-specific perturbations.Here, we report an extension of the RiboTag system, first developed to achieve cell type-restricted expression of epitope-tagged ribosomal protein (RPL22) in mouse tissue, to a variety of in vitro applications, including immortalized cell lines, primary mouse astrocytes, and hiPSC-derived hindigyanvishv.com neurons.

RiboTag expression enables depletion of up to 87 percent of off-target RNA in mixed species co-cultures.Nonetheless, depletion efficiency varies across independent experimental replicates, particularly for hiPSC-derived motor neurons.The challenges and potential of implementing RiboTags in complex in vitro cultures are discussed.